Iron-Gallic Acid Peptide Nanoparticles as a Versatile Platform for Cellular Delivery with Synergistic ROS Enhancement Effect

Cytosolic delivery of peptides is great interest owing to their biological functions, which could be utilized for therapeutic applications. However, susceptibility enzymatic degradation and multiple cellular barriers generally hinders clinical application. Integration into nanoparticles, can enhance the stability membrane permeability bioactive peptides, a promising strategy overcome extracellular intracellular obstacles. Herein, we present versatile platform various cargo by integration metallo-peptidic coordination nanoparticles. Both termini were conjugated with gallic acid (GA) assemble GA-modified nanostructures upon Fe(III). Initial pre-complexation Fe(III) poly-(vinylpolypyrrolidon) (PVP) as template favored formation are able deliver cells efficiently. Iron–gallic peptide nanoparticles (IGPNs) stable in water supposed generate reactive oxygen species (ROS) from endogenous H2O2 via Fenton reaction. The was successfully applied an exemplary set sequences varying length (1–7 amino acids) charge (negative, neutral, positive). To confirm capability transporting cargos cells, pro-apoptotic integrated IGPNs, demonstrated potent killing human cervix carcinoma HeLa murine neuroblastoma N2a at 10 µM concentration complementary mechanisms peptide-triggered apoptosis Fe(III)-mediated ROS generation. This study demonstrates establishment IGPNs novel assembly used combined intrinsic